Research Papers: Imaging

Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy

[+] Author Affiliations
Jing-Yu Huang, Lun-Zhang Guo, Jing-Zun Wang, Tse-Chung Li

National Taiwan University, Institute of Biomedical Engineering, Taipei, Taiwan

Hsin-Jung Lee, Lung-Han Peng

National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, Taiwan

Po-Kai Chiu

Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu, Taiwan

Tzu-Ming Liu

National Taiwan University, Institute of Biomedical Engineering, Taipei, Taiwan

University of Macau, Faculty of Health Sciences, Taipa, Macao SAR, China

National Taiwan University, Molecular Imaging Center, Taipei, Taiwan

J. Biomed. Opt. 22(3), 036008 (Mar 08, 2017). doi:10.1117/1.JBO.22.3.036008
History: Received September 18, 2016; Accepted February 3, 2017
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Abstract.  Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Jing-Yu Huang ; Lun-Zhang Guo ; Jing-Zun Wang ; Tse-Chung Li ; Hsin-Jung Lee, et al.
"Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy", J. Biomed. Opt. 22(3), 036008 (Mar 08, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.3.036008


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