Research Papers: Imaging

Ptychographic phase microscope based on high-speed modulation on the illumination beam

[+] Author Affiliations
Yudong Yao

Shanghai Institute of Optics and Fine Mechanics, Key Laboratory of High Power Laser and Physics, Chinese Academy of Sciences, Shanghai, China

National Laboratory on High Power Laser and Physics, Chinese Academy of Sciences, Shanghai, China

University of Chinese Academy of Sciences, Beijing, China

Suhas P. Veetil

Higher Colleges of Technology, Department of Engineering Technology and Science, Fujairah, United Arab Emirates

Cheng Liu, JianQiang Zhu

Shanghai Institute of Optics and Fine Mechanics, Key Laboratory of High Power Laser and Physics, Chinese Academy of Sciences, Shanghai, China

National Laboratory on High Power Laser and Physics, Chinese Academy of Sciences, Shanghai, China

J. Biomed. Opt. 22(3), 036010 (Mar 10, 2017). doi:10.1117/1.JBO.22.3.036010
History: Received September 12, 2016; Accepted February 22, 2017
Text Size: A A A

Abstract.  A type of ptychography-based phase microscope was developed by integrating a spatial light modulator (SLM) into a commercial wide-field light microscope. By displaying a moving pattern on the SLM to change the sample illumination and record the diffraction intensities formed, both the modulus and phase of the transmission function of the sample could be accurately reconstructed with formulas similar to those of common ptychography. Compared with other kinds of phase microscopes, the developed microscope has several advantages, including its simple structure, high immunity to coherent noise, and low requirement for quality optics. In addition, defects in the illumination beam are also removed from the reconstructed image. Further, this microscope’s fast data acquisition ability makes it highly suitable for many applications where highly accurate quantitative phase imaging is important, such as in living cells or other fragile biological samples that cannot sustain continuous imaging over a long period of time.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Yudong Yao ; Suhas P. Veetil ; Cheng Liu and JianQiang Zhu
"Ptychographic phase microscope based on high-speed modulation on the illumination beam", J. Biomed. Opt. 22(3), 036010 (Mar 10, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.3.036010


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.