Furthermore, to improve the learning curve and gain further acceptance, another approach is for the appearance of mosaics to be in colors and contrast that will be familiar. The CSM microscope will be able to acquire images with two contrast mechanisms, fluorescence and reflectance, simultaneously. This allows us to use a nuclear contrast agent to highlight the nuclei in a fluorescence image while acquiring a reflectance image of the cellular and dermal collagen structures of the specimen. The process simulates the staining mechanism of H&E histopathology where hematoxylin provides the nuclear contrast and eosin highlights the cytoplasm, connective tissue, and extracellular substances. The fluorescence image and the reflectance image will be combined and digitally colorized with purple and pink spectrum such that the appearance of the CSM mosaics emulates traditional H&E-stained histopathology. Therefore, we use the term digital-H&E (dH&E) to describe this process. Unlike many pseudocoloring techniques, which are “artificial” (i.e., purely digital, without a physical basis), our digital-H&E technique is based on biological and physical (optical) characteristics of the tissue and the staining process to draw contrast, similar to traditional H&E staining. Although a similar approach that was reported in our earlier work in imaging skin,26 we expect produce sharper images with the CSM microscope by correcting for chromatic shift between reflectance and fluorescence images. The integration of the mosaicking and dH&E approaches is in progress.