Research Papers: Imaging

Automatic Gleason grading of prostate cancer using quantitative phase imaging and machine learning

[+] Author Affiliations
Tan H. Nguyen, Shamira Sridharan, Gabriel Popescu

University of Illinois, Beckman Institute for Advanced Science and Technology, Department of Electrical and Computer Engineering, Quantitative Light Imaging Laboratory, Urbana–Champaign, Illinois, United States

Virgilia Macias, Andre Kajdacsy-Balla

University of Illinois, Department of Pathology, Chicago, Illinois, United States

Jonathan Melamed

New York University, School of Medicine, Department of Pathology, New York, New York, United States

Minh N. Do

University of Illinois, Department of Electrical and Computer Engineering, Computational Imaging Group, Coordinated Science Laboratory, Urbana–Champaign, Illinois, United States

J. Biomed. Opt. 22(3), 036015 (Mar 30, 2017). doi:10.1117/1.JBO.22.3.036015
History: Received January 12, 2017; Accepted March 13, 2017
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Abstract.  We present an approach for automatic diagnosis of tissue biopsies. Our methodology consists of a quantitative phase imaging tissue scanner and machine learning algorithms to process these data. We illustrate the performance by automatic Gleason grading of prostate specimens. The imaging system operates on the principle of interferometry and, as a result, reports on the nanoscale architecture of the unlabeled specimen. We use these data to train a random forest classifier to learn textural behaviors of prostate samples and classify each pixel in the image into different classes. Automatic diagnosis results were computed from the segmented regions. By combining morphological features with quantitative information from the glands and stroma, logistic regression was used to discriminate regions with Gleason grade 3 versus grade 4 cancer in prostatectomy tissue. The overall accuracy of this classification derived from a receiver operating curve was 82%, which is in the range of human error when interobserver variability is considered. We anticipate that our approach will provide a clinically objective and quantitative metric for Gleason grading, allowing us to corroborate results across instruments and laboratories and feed the computer algorithms for improved accuracy.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Tan H. Nguyen ; Shamira Sridharan ; Virgilia Macias ; Andre Kajdacsy-Balla ; Jonathan Melamed, et al.
"Automatic Gleason grading of prostate cancer using quantitative phase imaging and machine learning", J. Biomed. Opt. 22(3), 036015 (Mar 30, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.3.036015


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