Research Papers: Sensing

Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples

[+] Author Affiliations
Syed Abdul Mannan Kirmani

North Dakota State University, Electrical and Computer Engineering Department, Fargo, North Dakota, United States

COMSATS Institute of Information Technology, Center for Advanced Studies in Telecommunication, Islamabad, Pakistan

Fleming Dackson Gudagunti, Logeeshan Velmanickam, Dharmakeerthi Nawarathna, Ivan T. Lima, Jr.

North Dakota State University, Electrical and Computer Engineering Department, Fargo, North Dakota, United States

J. Biomed. Opt. 22(3), 037006 (Mar 30, 2017). doi:10.1117/1.JBO.22.3.037006
History: Received November 29, 2016; Accepted March 14, 2017
Text Size: A A A

Abstract.  We propose the use of negative dielectrophoresis (DEP) spectroscopy as a technique to improve the detection limit of rare analytes in biological samples. We observe a significant dependence of the negative DEP force on functionalized polystyrene beads at the edges of interdigitated electrodes with respect to the frequency of the electric field. We measured this velocity of repulsion for 0% and 0.8% conjugation of avidin with biotin functionalized polystyrene beads with our automated software through real-time image processing that monitors the Rayleigh scattering from the beads. A significant difference in the velocity of the beads was observed in the presence of as little as 80 molecules of avidin per biotin functionalized bead. This technology can be applied in the detection and quantification of rare analytes that can be useful in the diagnosis and the treatment of diseases, such as cancer and myocardial infarction, with the use of polystyrene beads functionalized with antibodies for the target biomarkers.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Syed Abdul Mannan Kirmani ; Fleming Dackson Gudagunti ; Logeeshan Velmanickam ; Dharmakeerthi Nawarathna and Ivan T. Lima, Jr.
"Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples", J. Biomed. Opt. 22(3), 037006 (Mar 30, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.3.037006


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.