Research Papers: Imaging

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

[+] Author Affiliations
Chenxi Li, Ruikang Wang

University of Washington, Department of Bioengineering, Seattle, Washington, United States

Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin, China

J. Biomed. Opt. 22(4), 046002 (Apr 06, 2017). doi:10.1117/1.JBO.22.4.046002
History: Received February 14, 2017; Accepted March 21, 2017
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Abstract.  We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Chenxi Li and Ruikang Wang
"Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal", J. Biomed. Opt. 22(4), 046002 (Apr 06, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.4.046002


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Blood flow determination by the laser speckle method. Int J Microcirc Clin Exp 1990;9(1):21-45.
Retinal blood flow evaluation. Ophthalmologica 2013;229(2):61-74.
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