JBO Letters

Fluorescence lifetime-based contrast enhancement of indocyanine green-labeled tumors

[+] Author Affiliations
Anand T. N. Kumar, Stefan A. Carp, Jing Yang, Alana Ross, Zdravka Medarova, Chongzhao Ran

Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States

J. Biomed. Opt. 22(4), 040501 (Apr 11, 2017). doi:10.1117/1.JBO.22.4.040501
History: Received January 28, 2017; Accepted March 21, 2017
Text Size: A A A

Abstract.  Although the development of tumor-targeted fluorescent probes is a major area of investigation, it will be several years before these probes are realized for clinical use. Here, we report an approach that employs indocyanine-green (ICG), a clinically approved, nontargeted dye, in conjunction with fluorescence lifetime (FLT) detection to provide high accuracy for tumor-tissue identification in mouse models of subcutaneous human breast and brain tmors. The improved performance relies on the distinct FLTs of ICG within tumors versus tissue autofluorescence and is further aided by the well-known enhanced permeability and retention of ICG in tumors and the clearance of ICG from normal tissue several hours after intravenous injection. We demonstrate that FLT detection can provide more than 98% sensitivity and specificity, and a 10-fold reduction in error rates compared to intensity-based detection. Our studies suggest the significant potential of FLT-contrast for accurate tumor-tissue identification using ICG and other targeted probes under development, both for intraoperative imaging and for ex-vivo margin assessment of surgical specimens.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Anand T. N. Kumar ; Stefan A. Carp ; Jing Yang ; Alana Ross ; Zdravka Medarova, et al.
"Fluorescence lifetime-based contrast enhancement of indocyanine green-labeled tumors", J. Biomed. Opt. 22(4), 040501 (Apr 11, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.4.040501


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.