Research Papers: Imaging

Near-infrared angiography for critical limb ischemia in a diabetic murine model

[+] Author Affiliations
Missael Garcia

Washington University, Department of Computer Science and Engineering, St. Louis, Missouri, United States

Mohamed A. Zayed

Washington University School of Medicine, Department of Surgery, Section of Vascular Surgery, St. Louis, Missouri, United States

Veterans Affairs St. Louis Health Care System, Department of Surgery, St. Louis, Missouri, United States

Kyoung-mi Park

Washington University School of Medicine, Department of Surgery, Section of Vascular Surgery, St. Louis, Missouri, United States

Viktor Gruev

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Urbana, Illinois, United States

University of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, Urbana, Illinois, United States

J. Biomed. Opt. 22(4), 046006 (Apr 18, 2017). doi:10.1117/1.JBO.22.4.046006
History: Received February 23, 2017; Accepted March 30, 2017
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Abstract.  Peripheral arterial disease (PAD) is a highly prevalent disease process that afflicts more than 20% of individuals with diabetes. Progression of PAD in the setting of diabetes can lead to critical limb ischemia (CLI), which is associated with increased risk of wounds, gangrene, and limb loss. Prompt noninvasive evaluation of limbs affected by PAD progression and CLI is currently limited. Here, we evaluate the utility of a custom-designed multispectral imaging system for fluorescence-based near-infrared angiography and compare it to the existing gold standard of laser-scanning Doppler perfusion assessments. Due to its higher resolution and fluorescence sensitivity, near-infrared angiography demonstrates a greater capacity to characterize altered dynamic arterial perfusion in a clinically relevant diabetic murine model for CLI. Furthermore, we demonstrate that our imaging system can accurately track arterial perfusion recovery over time following induced ischemia, and reveal unique phenotypic differences in the setting of diabetes.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Missael Garcia ; Mohamed A. Zayed ; Kyoung-mi Park and Viktor Gruev
"Near-infrared angiography for critical limb ischemia in a diabetic murine model", J. Biomed. Opt. 22(4), 046006 (Apr 18, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.4.046006


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