Special Section on Advanced Laser Technologies for Biophotonics

Assessment of decellularization of heart bioimplants using a Raman spectroscopy method

[+] Author Affiliations
Elena V. Timchenko, Pavel E. Timchenko, Svetlana V. Pershutkina

Samara National Research University, Samara, Russia

Artur Lichtenberg, Alexander Assmann, Hug Aubin, Payam Akhyari

Heinrich Heine University, Clinic of Cardiovascular Surgery, Dusseldorf, Germany

Larisa T. Volova

Experimental Medicine and Biotechnologies Institute of the Samara State Medical University, Samara, Russia

J. Biomed. Opt. 22(9), 091511 (Apr 18, 2017). doi:10.1117/1.JBO.22.9.091511
History: Received December 3, 2016; Accepted March 22, 2017
Text Size: A A A

Abstract.  We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Elena V. Timchenko ; Pavel E. Timchenko ; Artur Lichtenberg ; Alexander Assmann ; Hug Aubin, et al.
"Assessment of decellularization of heart bioimplants using a Raman spectroscopy method", J. Biomed. Opt. 22(9), 091511 (Apr 18, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.9.091511


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.