Research Papers: Imaging

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

[+] Author Affiliations
Daniel Baruch

Ariel University, Department of Electrical and Electronics Engineering, Ariel, Israel

Ariel University, Department of Physics, Ariel, Israel

David Abookasis

Ariel University, Department of Electrical and Electronics Engineering, Ariel, Israel

J. Biomed. Opt. 22(4), 046007 (Apr 20, 2017). doi:10.1117/1.JBO.22.4.046007
History: Received November 17, 2016; Accepted March 29, 2017
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Abstract.  The application of optical techniques as tools for biomedical research has generated substantial interest for the ability of such methodologies to simultaneously measure biochemical and morphological parameters of tissue. Ongoing optimization of optical techniques may introduce such tools as alternative or complementary to conventional methodologies. The common approach shared by current optical techniques lies in the independent acquisition of tissue’s optical properties (i.e., absorption and reduced scattering coefficients) from reflected or transmitted light. Such optical parameters, in turn, provide detailed information regarding both the concentrations of clinically relevant chromophores and macroscopic structural variations in tissue. We couple a noncontact optical setup with a simple analysis algorithm to obtain absorption and scattering coefficients of biological samples under test. Technically, a portable picoprojector projects serial sinusoidal patterns at low and high spatial frequencies, while a spectrometer and two independent CCD cameras simultaneously acquire the reflected diffuse light through a single spectrometer and two separate CCD cameras having different bandpass filters at nonisosbestic and isosbestic wavelengths in front of each. This configuration fills the gaps in each other’s capabilities for acquiring optical properties of tissue at high spectral and spatial resolution. Experiments were performed on both tissue-mimicking phantoms as well as hands of healthy human volunteers to quantify their optical properties as proof of concept for the present technique. In a separate experiment, we derived the optical properties of the hand skin from the measured diffuse reflectance, based on a recently developed camera model. Additionally, oxygen saturation levels of tissue measured by the system were found to agree well with reference values. Taken together, the present results demonstrate the potential of this integrated setup for diagnostic and research applications.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Daniel Baruch and David Abookasis
"Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination", J. Biomed. Opt. 22(4), 046007 (Apr 20, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.4.046007


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