Special Section on Advanced Laser Technologies for Biophotonics

Off-axis holographic laser speckle contrast imaging of blood vessels in tissues

[+] Author Affiliations
Arkady Abdurashitov

Saratov National Research State University, Research-Educational Institute of Optics and Biophotonics, Saratov, Russia

Olga Bragina

Saratov National Research State University, Department of Human and Animal Physiology, Saratov, Russia

University of New Mexico School of Medicine, Department of Neurosurgery, Albuquerque, New Mexico, United States

Olga Sindeeva

Saratov National Research State University, Department of Human and Animal Physiology, Saratov, Russia

Queen Mary University of London, School of Engineering and Materials Science, London, United Kingdom

Saratov National Research State University, Remote Controlled Theranostic Systems Laboratory, Saratov, Russia

Sindeev Sergey, Oxana V. Semyachkina-Glushkovskaya

Saratov National Research State University, Department of Human and Animal Physiology, Saratov, Russia

Valery V. Tuchin

Saratov National Research State University, Research-Educational Institute of Optics and Biophotonics, Saratov, Russia

Institute of Precision Mechanics and Control RAS, Laboratory of Laser Diagnostics of Technical and Living Systems, Saratov, Russia

National Research Tomsk State University, Interdisciplinary Laboratory of Biophotonics, Tomsk, Russia

J. Biomed. Opt. 22(9), 091514 (Apr 25, 2017). doi:10.1117/1.JBO.22.9.091514
History: Received December 31, 2016; Accepted March 27, 2017
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Abstract.  Laser speckle contrast imaging (LSCI) has become one of the most common tools for functional imaging in tissues. Incomplete theoretical description and sophisticated interpretation of measurement results are completely sidelined by a low-cost and simple hardware, fastness, consistent results, and repeatability. In addition to the relatively low measuring volume with around 700  μm of the probing depth for the visible spectral range of illumination, there is no depth selectivity in conventional LSCI configuration; furthermore, in a case of high NA objective, the actual penetration depth of light in tissues is greater than depth of field (DOF) of an imaging system. Thus, the information about these out-of-focus regions persists in the recorded frames but cannot be retrieved due to intensity-based registration method. We propose a simple modification of LSCI system based on the off-axis holography to introduce after-registration refocusing ability to overcome both depth-selectivity and DOF problems as well as to get the potential possibility of producing a cross-section view of the specimen.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Arkady Abdurashitov ; Olga Bragina ; Olga Sindeeva ; Sindeev Sergey ; Oxana V. Semyachkina-Glushkovskaya, et al.
"Off-axis holographic laser speckle contrast imaging of blood vessels in tissues", J. Biomed. Opt. 22(9), 091514 (Apr 25, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.9.091514


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