Research Papers: General

Evidence of anti-inflammatory effect and percutaneous penetration of a topically applied fish oil preparation: a photoacoustic spectroscopy study

[+] Author Affiliations
Franciele Q. Ames, Laura L. M. de Arruda, Bruno A. da Rocha, Roberto K. N. Cuman, Ciomar A. Bersani-Amado

State University of Maringá, Department of Pharmacology and Therapeutics, Maringá, Paraná, Brazil

Francielle Sato, Lidiane V. de Castro, Mauro L. Baesso

State University of Maringá, Department of Physics, Maringá, Paraná, Brazil

J. Biomed. Opt. 22(5), 055003 (May 10, 2017). doi:10.1117/1.JBO.22.5.055003
History: Received December 1, 2016; Accepted April 19, 2017
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Abstract.  This paper investigates the topical anti-inflammatory effect of a fish oil preparation (FOP) in a croton oil (CO) model of skin inflammation. The photoacoustic spectroscopy (PAS) was applied to estimate the percutaneous penetration of the FOP and as a model to evaluate the topical inflammatory response. After applying CO, the groups of mice received a topical application of a FOP on the left ear. The right ear received the vehicle that was used to dilute the CO. After 6 h, ear tissue was collected to determine the percent inhibition of edema, myeloperoxidase (MPO) activity, and cytokine levels and to perform PAS measurements. Treatment with FOP reduced edema and MPO activity, which was at least partially attributed to a decrease in the levels of tumor necrosis factor, interleukin-1β, interleukin-6, keratinocyte-derived chemokine, and monocyte chemoattractant protein-1. The topically applied FOP penetrated into the tissue and decreased the area of the bands that characterize inflamed tissue. The present results demonstrated the topical anti-inflammatory effect of the FOP. PAS suggests that FOP anti-inflammatory activity is linked with its ability to penetrate through the skin.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Franciele Q. Ames ; Francielle Sato ; Lidiane V. de Castro ; Laura L. M. de Arruda ; Bruno A. da Rocha, et al.
"Evidence of anti-inflammatory effect and percutaneous penetration of a topically applied fish oil preparation: a photoacoustic spectroscopy study", J. Biomed. Opt. 22(5), 055003 (May 10, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.5.055003


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