Research Papers: Imaging

Sensing of Streptococcus mutans by microscopic imaging ellipsometry

[+] Author Affiliations
Mai Ibrahim Khaleel, Yu-Da Chen, Ching-Hang Chien

Academia Sinica, Research Center for Applied Sciences, Taipei, Taiwan

Academia Sinica and National Tsing Hua University, Nano Science and Technology Program, Taiwan International Graduate Program, Taiwan

National Tsing Hua University, Department of Engineering and System Science, Hsinchu, Taiwan

Yia-Chung Chang

Academia Sinica, Research Center for Applied Sciences, Taipei, Taiwan

J. Biomed. Opt. 22(5), 056005 (May 16, 2017). doi:10.1117/1.JBO.22.5.056005
History: Received January 29, 2017; Accepted April 21, 2017
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Abstract.  Microscopic imaging ellipsometry is an optical technique that uses an objective and sensing procedure to measure the ellipsometric parameters Ψ and Δ in the form of microscopic maps. This technique is well known for being noninvasive and label-free. Therefore, it can be used to detect and characterize biological species without any impact. Microscopic imaging ellipsometry was used to measure the optical response of dried Streptococcus mutans cells on a glass substrate. The ellipsometric Ψ and Δ maps were obtained with the Optrel Multiskop system for specular reflection in the visible range (λ=450 to 750 nm). The Ψ and Δ images at 500, 600, and 700 nm were analyzed using three different theoretical models with single-bounce, two-bounce, and multibounce light paths to obtain the optical constants and height distribution. The obtained images of the optical constants show different aspects when comparing the single-bounce analysis with the two-bounce or multibounce analysis in detecting S. mutans samples. Furthermore, the height distributions estimated by two-bounce and multibounce analyses of S. mutans samples were in agreement with the thickness values measured by AFM, which implies that the two-bounce and multibounce analyses can provide information complementary to that obtained by a single-bounce light path.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Mai Ibrahim Khaleel ; Yu-Da Chen ; Ching-Hang Chien and Yia-Chung Chang
"Sensing of Streptococcus mutans by microscopic imaging ellipsometry", J. Biomed. Opt. 22(5), 056005 (May 16, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.5.056005


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