Research Papers: General

Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green

[+] Author Affiliations
Irina Yu. Yanina

Saratov National Research State University, Research-Educational Institute of Optics and Biophotonics, Saratov, Russia

National Research Tomsk State University, Interdisciplinary Laboratory of Biophotonics, Tomsk, Russia

Educational and Research Institute of Nanostructures and Biosystems, Remote Controlled Theranostic Systems Lab, Saratov, Russia

Nikita A. Navolokin

Educational and Research Institute of Nanostructures and Biosystems, Remote Controlled Theranostic Systems Lab, Saratov, Russia

Saratov State Medical University, Saratov, Russia

Yulia I. Svenskaya, Dmitry A. Gorin

Educational and Research Institute of Nanostructures and Biosystems, Remote Controlled Theranostic Systems Lab, Saratov, Russia

Alla B. Bucharskaya, Galina N. Maslyakova

Saratov State Medical University, Saratov, Russia

Gleb B. Sukhorukov

Educational and Research Institute of Nanostructures and Biosystems, Remote Controlled Theranostic Systems Lab, Saratov, Russia

Queen Mary University of London, School of Engineering and Materials Sciences, London, United Kingdom

Valery V. Tuchin

Saratov National Research State University, Research-Educational Institute of Optics and Biophotonics, Saratov, Russia

National Research Tomsk State University, Interdisciplinary Laboratory of Biophotonics, Tomsk, Russia

Institute of the Russian Academy of Sciences, Laboratory of Laser Diagnostics of Technical and Living Systems of Precision Mechanics and Control, Saratov, Russia

J. Biomed. Opt. 22(5), 055008 (May 25, 2017). doi:10.1117/1.JBO.22.5.055008
History: Received January 27, 2017; Accepted May 2, 2017
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Abstract.  The goal of this study is to quantify the impact of the in vivo photochemical treatment of rats with obesity using indocyanine green (ICG) dissolved in saline or dispersed in an encapsulated form at NIR laser irradiation, which was monitored by tissue sampling and histochemistry. The subcutaneous injection of the ICG solution or ICG encapsulated into polyelectrolyte microcapsules, followed by diode laser irradiation (808 nm, 8  W/cm2, 1 min), resulted in substantial differences in lipolysis of subcutaneous fat. Most of the morphology alterations occurred in response to the laser irradiation if a free-ICG solution had been injected. In such conditions, membrane disruption, stretching, and even delamination in some cases were observed for a number of cells. The encapsulated ICG aroused similar morphology changes but with weakly expressed adipocyte destruction under the laser irradiation. The Cochran Q test rendered the difference between the treatment alternatives statistically significant. By this means, laser treatment using the encapsulated form of ICG seems more promising and could be used for safe layerwise laser treatment of obesity and cellulite.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Irina Yu. Yanina ; Nikita A. Navolokin ; Yulia I. Svenskaya ; Alla B. Bucharskaya ; Galina N. Maslyakova, et al.
"Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green", J. Biomed. Opt. 22(5), 055008 (May 25, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.5.055008


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