Special Section on Advanced Laser Technologies for Biophotonics

Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics

[+] Author Affiliations
Kisung Lee

Saarland University, Experimental Physics, Saarbrücken, Germany

Lomonosov Moscow State University, Faculty of Physics, Moscow, Russia

Christian Wagner

Saarland University, Experimental Physics, Saarbrücken, Germany

University of Luxembourg, Physics and Materials Science Research Unit, Luxembourg

Alexander V. Priezzhev

Lomonosov Moscow State University, Faculty of Physics, Moscow, Russia

Lomonosov Moscow State University, International Laser Center, Moscow, Russia

J. Biomed. Opt. 22(9), 091516 (Jun 21, 2017). doi:10.1117/1.JBO.22.9.091516
History: Received December 1, 2016; Accepted May 26, 2017
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Abstract.  Red blood cell (RBC) aggregation is an intrinsic property of the blood that has a direct effect on the blood viscosity and circulation. Nevertheless, the mechanism behind the RBC aggregation has not been confirmed and is still under investigation with two major hypotheses, known as “depletion layer” and “cross-bridging.” We aim to ultimately understand the mechanism of the RBC aggregation and clarify both models. To measure the cell interaction in vitro in different suspensions (including plasma, isotonic solution of fibrinogen, isotonic solution of fibrinogen with albumin, and phosphate buffer saline) while moving the aggregate from one solution to another, an approach combining optical trapping and microfluidics has been applied. The study reveals evidence that RBC aggregation in plasma is at least partly due to the cross-bridging mechanism. The cell interaction strength measured in the final solution was found to be significantly changed depending on the initial solution where the aggregate was formed.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Kisung Lee ; Christian Wagner and Alexander V. Priezzhev
"Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics", J. Biomed. Opt. 22(9), 091516 (Jun 21, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.9.091516


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