Research Papers: Imaging

Dynamic measurements of flowing cells labeled by gold nanoparticles using full-field photothermal interferometric imaging

[+] Author Affiliations
Nir A. Turko, Darina Roitshtain, Omry Blum, Natan T. Shaked

Tel Aviv University, Department of Biomedical Engineering, Tel Aviv, Israel

Björn Kemper

University of Münster, Biomedical Technology Center, Münster, Germany

J. Biomed. Opt. 22(6), 066012 (Jun 21, 2017). doi:10.1117/1.JBO.22.6.066012
History: Received February 17, 2017; Accepted May 25, 2017
Text Size: A A A

Abstract.  We present highly dynamic photothermal interferometric phase microscopy for quantitative, selective contrast imaging of live cells during flow. Gold nanoparticles can be biofunctionalized to bind to specific cells, and stimulated for local temperature increase due to plasmon resonance, causing a rapid change of the optical phase. These phase changes can be recorded by interferometric phase microscopy and analyzed to form an image of the binding sites of the nanoparticles in the cells, gaining molecular specificity. Since the nanoparticle excitation frequency might overlap with the sample dynamics frequencies, photothermal phase imaging was performed on stationary or slowly dynamic samples. Furthermore, the computational analysis of the photothermal signals is time consuming. This makes photothermal imaging unsuitable for applications requiring dynamic imaging or real-time analysis, such as analyzing and sorting cells during fast flow. To overcome these drawbacks, we utilized an external interferometric module and developed new algorithms, based on discrete Fourier transform variants, enabling fast analysis of photothermal signals in highly dynamic live cells. Due to the self-interference module, the cells are imaged with and without excitation in video-rate, effectively increasing signal-to-noise ratio. Our approach holds potential for using photothermal cell imaging and depletion in flow cytometry.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Nir A. Turko ; Darina Roitshtain ; Omry Blum ; Björn Kemper and Natan T. Shaked
"Dynamic measurements of flowing cells labeled by gold nanoparticles using full-field photothermal interferometric imaging", J. Biomed. Opt. 22(6), 066012 (Jun 21, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.6.066012


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.