Research Papers: Imaging

Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells

[+] Author Affiliations
Aisada Uchugonova

Saarland University, Department of Biophotonics and Laser Technology, Saarbrücken, Germany

JenLab GmbH, Jena, Germany

J. Biomed. Opt. 22(6), 066018 (Jun 30, 2017). doi:10.1117/1.JBO.22.6.066018
History: Received February 23, 2017; Accepted June 7, 2017
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Abstract.  The multiphoton fluorescence lifetime imaging tomograph MPTflex with its flexible 360-deg scan head, articulated arm, and tunable femtosecond laser source was employed to study induced pluripotent stem cell (iPS) cultures. Autofluorescence (AF) lifetime imaging was performed with 250-ps temporal resolution and submicron spatial resolution using time-correlated single-photon counting. The two-photon excited AF was based on the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide/flavoproteins. iPS cells generated from mouse embryonic fibroblasts (MEFs) and cocultured with growth-arrested MEFs as feeder cells have been studied. Significant differences on AF lifetime signatures were identified between iPS and feeder cells as well as between their differentiating counterparts.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Aisada Uchugonova
"Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells", J. Biomed. Opt. 22(6), 066018 (Jun 30, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.6.066018


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