Research Papers: Imaging

Preclinical evaluation of spatial frequency domain-enabled wide-field quantitative imaging for enhanced glioma resection

[+] Author Affiliations
Mira Sibai, Carl Fisher, Brian C. Wilson

University of Toronto, Department of Medical Biophysics, Faculty of Medicine, Ontario, Canada

University Health Network, Princess Margaret Cancer Center, Ontario, Canada

Israel Veilleux

University Health Network, Princess Margaret Cancer Center, Ontario, Canada

Jonathan T. Elliott

Dartmouth College, Thayer School of Engineering, New Hampshire, United States

Frederic Leblond

École Polytechnique De Montreal, Department of Engineering Physics, Québec, Canada

David W. Roberts

Dartmouth Hitchcock Medical Center, Department of Neurosurgery, New Hampshire, United States

J. Biomed. Opt. 22(7), 076007 (Jul 11, 2017). doi:10.1117/1.JBO.22.7.076007
History: Received January 23, 2017; Accepted June 21, 2017
Text Size: A A A

Abstract.  5-Aminolevelunic acid-induced protoporphyrin IX (PpIX) fluorescence-guided resection (FGR) enables maximum safe resection of glioma by providing real-time tumor contrast. However, the subjective visual assessment and the variable intrinsic optical attenuation of tissue limit this technique to reliably delineating only high-grade tumors that display strong fluorescence. We have previously shown, using a fiber-optic probe, that quantitative assessment using noninvasive point spectroscopic measurements of the absolute PpIX concentration in tissue further improves the accuracy of FGR, extending it to surgically curable low-grade glioma. More recently, we have shown that implementing spatial frequency domain imaging with a fluorescent-light transport model enables recovery of two-dimensional images of [PpIX], alleviating the need for time-consuming point sampling of the brain surface. We present first results of this technique modified for in vivo imaging on an RG2 rat brain tumor model. Despite the moderate errors in retrieving the absorption and reduced scattering coefficients in the subdiffusive regime of 14% and 19%, respectively, the recovered [PpIX] maps agree within 10% of the point [PpIX] values measured by the fiber-optic probe, validating its potential as an extension or an alternative to point sampling during glioma resection.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Mira Sibai ; Carl Fisher ; Israel Veilleux ; Jonathan T. Elliott ; Frederic Leblond, et al.
"Preclinical evaluation of spatial frequency domain-enabled wide-field quantitative imaging for enhanced glioma resection", J. Biomed. Opt. 22(7), 076007 (Jul 11, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.7.076007


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.