Research Papers: Imaging

Can photoacoustic imaging quantify surface-localized J-aggregating nanoparticles?

[+] Author Affiliations
Liang Lim, Robert Mastragostino

University Health Network, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

Kenneth Ng

University of Toronto, Department of Medical Biophysics, Toronto, Ontario, Canada

Gang Zheng, Brian C. Wilson

University Health Network, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

University of Toronto, Department of Medical Biophysics, Toronto, Ontario, Canada

J. Biomed. Opt. 22(7), 076008 (Jul 13, 2017). doi:10.1117/1.JBO.22.7.076008
History: Received January 16, 2017; Accepted June 21, 2017
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Abstract.  We investigate the feasibility of photoacoustic (PA) imaging to quantify the concentration of surface-localized nanoparticles, using tissue-mimicking phantoms and imaging with a commercial PA instrument at 815 nm and a linear-array transducer at a center frequency of 40 MHz. The nanoparticles were J-aggregating porphysomes (JNP) comprising self-assembling, all-organic porphyrin-lipid micelles with a molar absorption coefficient of 8.7×108  cm1M1 at this wavelength. The PA signal intensity versus JNP areal concentration followed a sigmoidal curve with a reproducible linear range of 17  fmol/mm2 to 11  pmol/mm2, i.e., 3 orders of magnitude with ±34% error. For physiologically-relevant conditions (i.e., optical scattering-dominated tissues: transport albedo >0.8) and JNP concentrations above 330  fmol/mm2, the PA signal depends only on the nanoparticle concentration. Otherwise, independent measurement of the optical absorption and scattering properties of the underlying tissue is required for accurate quantification. The implications for surface PA imaging, such as in the use of targeted nanoparticles applied topically to tissue as in endoscopic diagnosis, are considered.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Liang Lim ; Robert Mastragostino ; Kenneth Ng ; Gang Zheng and Brian C. Wilson
"Can photoacoustic imaging quantify surface-localized J-aggregating nanoparticles?", J. Biomed. Opt. 22(7), 076008 (Jul 13, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.7.076008


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