Super-multiplexed vibrational imaging for 3D spatial biology

Lixue Shi; Wei Min

doi:10.1117/12.2607391

7 March 2022 Super-multiplexed vibrational imaging for 3D spatial biology

Lixue Shi, Wei Min

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC119650O (2022) https://doi.org/10.1117/12.2607391
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Understanding complex biological systems requires simultaneous characterization of a large number of interacting components in their native 3D environment. However, fluorescence methods are usually hindered by the fundamental color barrier. Here we will introduce electronic pre-resonance stimulated Raman scattering (epr-SRS) microscopy, an emerging method of ultrasensitive vibrational imaging with high potential of multiplexing. To generalize epr-SRS to large-scale volumetric imaging, we further integrated it with tissue clearing technology and achieved simultaneous visualization of >10 protein targets over millimeter thickness of brain tissues. Overall, super-multiplexed vibrational imaging is a promising tool to provide a complete picture of tissue biology.

Conference Presentation

Citation Download Citation

Lixue Shi and Wei Min "Super-multiplexed vibrational imaging for 3D spatial biology", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC119650O (7 March 2022); https://doi.org/10.1117/12.2607391

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