Frequency-modulation stimulated Raman scattering microscopy with an acousto-optic tunable filter.

Elisa Grassi; Siarhei Laptenok; Luca Genchi; Carlo Liberale

doi:10.1117/12.2608693

2 March 2022 Frequency-modulation stimulated Raman scattering microscopy with an acousto-optic tunable filter.

Elisa Grassi, Siarhei Laptenok, Luca Genchi, Carlo Liberale

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730U (2022) https://doi.org/10.1117/12.2608693
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Stimulated Raman Scattering (SRS) is a label-free technique utilized to analyze the chemical nature of various substances with high-speed. However, SRS can be affected by parasitic effects that can distort the signal. In order to eliminate these spurious effects, we propose a frequency-modulation SRS scheme realized for the first time with an acousto-optic tunable filter, which can perform measurements from the fingerprint to the CH-stretch region of the spectrum without any modification of the optical setup. We demonstrate the efficient cancellation of the parasitic effects with our developed configuration on several samples: human hairs, polymer beads and human cells.

Conference Presentation

Citation Download Citation

Elisa Grassi, Siarhei Laptenok, Luca Genchi, and Carlo Liberale "Frequency-modulation stimulated Raman scattering microscopy with an acousto-optic tunable filter.", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730U (2 March 2022); https://doi.org/10.1117/12.2608693

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