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2 March 2022 Slit-scanning Raman microscopy using Bessel beam illumination
Katsumasa Fujita, Kota Koike
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730B (2022) https://doi.org/10.1117/12.2609733
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We developed a Raman scattering microscope using a 0th-order Bessel beam to illuminate the inside of a biological sample. We constructed a Raman spectroscopic system in which the Bessel beam illuminates the sample from the side, and the illuminated area in the sample is imaged on the slit of a spectrophotometer. By using the developed microscope, polymer beads and biological samples were observed to confirm the imaging property and the improvement of the image contrast compared to the conventional line-illumination Raman microscopy.
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Katsumasa Fujita and Kota Koike "Slit-scanning Raman microscopy using Bessel beam illumination", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730B (2 March 2022); https://doi.org/10.1117/12.2609733
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KEYWORDS
Raman spectroscopy
Bessel beams
Microscopy
Raman scattering
Imaging spectroscopy
Spatial resolution
Spectroscopy
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