Motion-immune digital refocusing of point-scanning optical coherence tomography using Lissajous scan for in vivo imaging

Shuichi Makita; Lida Zhu; Pradipta Mukherjee; Yiheng Lim; Rion Morishita; Ibrahim Abd El-Sadek; Yoshiaki Yasuno

doi:10.1117/12.2647850

9 March 2023 Motion-immune digital refocusing of point-scanning optical coherence tomography using Lissajous scan for in vivo imaging

Shuichi Makita, Lida Zhu, Pradipta Mukherjee, Yiheng Lim, Rion Morishita, Ibrahim Abd El-Sadek, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123670M (2023) https://doi.org/10.1117/12.2647850
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Digital refocusing of optical coherence tomography (OCT) restores the spatial resolution degraded by defocusing. To apply this to imaging of biological tissue with point-scan Fourier domain OCT (FD-OCT), fast volume acquisition is needed to prevent motion disturbance. This study demonstrates the Lissajous-cycle-wise (LCW) digital refocusing algorithm applied to in vivo imaging at acquisition rates typical of FD-OCT. Blurring along and perpendicular to the scanning direction are compensated by inverse filtering and compositing A-lines of different scanning directions after motion correction, respectively. Ex vivo and in vivo biological sample experiments are applied for the proof of concept. The blurred images due to defocusing are sharpened by the LCW digital refocus algorithm.

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Shuichi Makita, Lida Zhu, Pradipta Mukherjee, Yiheng Lim, Rion Morishita, Ibrahim Abd El-Sadek, and Yoshiaki Yasuno "Motion-immune digital refocusing of point-scanning optical coherence tomography using Lissajous scan for in vivo imaging", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123670M (9 March 2023); https://doi.org/10.1117/12.2647850

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