Single-objective tilted light sheet illumination with exchange-PAINT and deep learning for fast, accurate, and precise 3D single-molecule super-resolution imaging in mammalian cells (Conference Presentation)

Gabriella P. Gagliano; Nahima Saliba; Armando Amador; Anna-Karin Gustavsson

doi:10.1117/12.2647406

15 March 2023 Single-objective tilted light sheet illumination with exchange-PAINT and deep learning for fast, accurate, and precise 3D single-molecule super-resolution imaging in mammalian cells (Conference Presentation)

Gabriella P. Gagliano, Nahima Saliba, Armando Amador, Anna-Karin Gustavsson

Author Affiliations +

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC1238607 (2023) https://doi.org/10.1117/12.2647406
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Super-resolution fluorescence microscopy for imaging in thick samples would benefit greatly from a reduction in fluorescence background and an increase in acquisition speed. To address these concerns, we present a single-objective tilted light sheet combined with four additional innovations: (i) a 3D-printed microfluidic chip, (ii) sequential DNA Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) known as Exchange-PAINT, (iii) deep learning for improved localization precision and imaging speeds, and (iv) engineered point spread functions for 3D imaging. Our approach achieves improved localization precision, imaging speeds, and multi-target accuracy, enabling fast and precise multi-target 3D super-resolution imaging.

Conference Presentation

Citation Download Citation

Gabriella P. Gagliano, Nahima Saliba, Armando Amador, and Anna-Karin Gustavsson "Single-objective tilted light sheet illumination with exchange-PAINT and deep learning for fast, accurate, and precise 3D single-molecule super-resolution imaging in mammalian cells (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC1238607 (15 March 2023); https://doi.org/10.1117/12.2647406

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