Practical and cost-effective implementations of fluorescence lifetime imaging systems (Conference Presentation)

Michael J. Serafino; Javier A. Jo

doi:10.1117/12.2647982

16 March 2023 Practical and cost-effective implementations of fluorescence lifetime imaging systems (Conference Presentation)

Michael J. Serafino, Javier A. Jo

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239103 (2023) https://doi.org/10.1117/12.2647982
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a novel, cost-effective, versatile, and practical frequency-domain fluorescence lifetime imaging/microscopy (FD-FLIM) implementation capable of simultaneous multi-wavelength excitation, simultaneous multispectral detection, and sub-nanosecond to nanosecond fluorescence lifetime estimation. Rigorous validation experiments have demonstrated the capabilities of this novel FD-FLIM implementation to accurately measure fluorescence lifetimes in the range of ~0.4-12 ns. This novel technology has been adopted to implement an FD-FLIM endoscopy system for label-free metabolic imaging of oral lesions based on NADH (375-nm excitation, 484/37-nm emission) and FAD (445-nm excitation, 553/93-nm emission) autofluorescence imaging. Future efforts will focus on developing imaging systems for simultaneous endogenous (UV-VI excitation) and exogenous (NIR excitation) fluorescence lifetime imaging.

Conference Presentation

Citation Download Citation

Michael J. Serafino and Javier A. Jo "Practical and cost-effective implementations of fluorescence lifetime imaging systems (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239103 (16 March 2023); https://doi.org/10.1117/12.2647982

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