Broadband fluorescence-encoded vibrational imaging (Conference Presentation)

Phillip C. McCann; Kotaro Hiramatsu; Keisuke Goda

doi:10.1117/12.2655732

15 March 2023 Broadband fluorescence-encoded vibrational imaging (Conference Presentation)

Phillip C. McCann, Kotaro Hiramatsu, Keisuke Goda

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239219 (2023) https://doi.org/10.1117/12.2655732
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS) is a fluorescence-encoded vibrational spectroscopy technique that boasts both high sensitivity and specificity in the probing of molecular vibrations in the lower fingerprint region. However, to date, all presented data presented so far has been from flowing samples which does not sufficiently demonstrate the method’s applicability for monitoring biological and chemical processes. To amend this, we demonstrate here FLETCHERS imaging of low-concentration samples with an expanded spectral window achieved by utilizing spatial filtering and the confocal collection of fluorescence-encoded light.

Conference Presentation

Citation Download Citation

Phillip C. McCann, Kotaro Hiramatsu, and Keisuke Goda "Broadband fluorescence-encoded vibrational imaging (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239219 (15 March 2023); https://doi.org/10.1117/12.2655732

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