Macroscopy fluorescence lifetime FRET monitors drug-target engagement in tumors in vivo

Amit Verma; Catherine Sherry; Vikas Pandey; Nanxue Yuan; John Williams; Xavier Intes; Margarida M. Barroso

doi:10.1117/12.3008749

13 March 2024 Macroscopy fluorescence lifetime FRET monitors drug-target engagement in tumors in vivo

Amit Verma, Catherine Sherry, Vikas Pandey, Nanxue Yuan, John Williams, Xavier Intes, Margarida M. Barroso

Proceedings Volume PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII; PC128210F (2024) https://doi.org/10.1117/12.3008749
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Macroscopic fluorescence lifetime Forster resonance energy transfer (MFLI FRET) imaging presents an analytical tool to non-invasively quantify drug-receptor engagement in tumors and other organs in preclinical studies. Near infrared (NIR) fluorescence lifetime FRET acts as a direct reporter of drug-target engagement via measuring the fraction of labeled-donor antibody drugs undergoing binding to their respective target receptor using in vitro microscopy or in vivo macroscopy. We show that NIR MFLI FRET measurements correlates with drug-receptor binding in tumor cells, but strikingly, not with ubiquitously used ex vivo receptor expression assessment. Our study demonstrates that MFLI FRET is a powerful non-invasive imaging approach to study the impact of tumor microenvironment on drug delivery and drug-target engagement in intact live tumor xenografts.

Conference Presentation

Citation Download Citation

Amit Verma, Catherine Sherry, Vikas Pandey, Nanxue Yuan, John Williams, Xavier Intes, and Margarida M. Barroso "Macroscopy fluorescence lifetime FRET monitors drug-target engagement in tumors in vivo", Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC128210F (13 March 2024); https://doi.org/10.1117/12.3008749

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available