Imaging of nanoparticle-labeled live cells in 3D culture with photothermal optical coherence tomography (OCT)

Jingyu Sun; Tianqi Fang; Hongjun Wang; Shang Wang

doi:10.1117/12.3006228

13 March 2024 Imaging of nanoparticle-labeled live cells in 3D culture with photothermal optical coherence tomography (OCT)

Jingyu Sun, Tianqi Fang, Hongjun Wang, Shang Wang

Author Affiliations +

Proceedings Volume PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI; PC1284109 (2024) https://doi.org/10.1117/12.3006228
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present mesoscale imaging of nanoparticle-labeled cells in 3D culture with photothermal optical coherence tomography (PT-OCT). Cells labeled with gold nanorods (AuNRs) are encapsulated in agarose-based gel for PT-OCT with 660-nm laser. We show a binary PT contrast for single cell detection without causing cell death. We also demonstrate 3D mapping of AuNR-labeled cells within optically scattering media by imaging through a layer of polystyrene beads mimicking highly scattering tissue and through a layer of cells without AuNRs. This method has an imaging scale that complements the other major cell imaging techniques for studying live cells in 3D culture.

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Jingyu Sun, Tianqi Fang, Hongjun Wang, and Shang Wang "Imaging of nanoparticle-labeled live cells in 3D culture with photothermal optical coherence tomography (OCT)", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC1284109 (13 March 2024); https://doi.org/10.1117/12.3006228

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KEYWORDS

Biological imaging

3D image processing

Live cell imaging

Optical coherence tomography

3D acquisition

Fluorescence imaging

Modulation

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