Imaging of single retinal ganglion cell with differential interference contrast microscopy (Conference Presentation)

Juyeong Oh; Yu Jeong Kim; Chul-Ki Kim; Taik Jin Lee; Mina Seo; Seok Lee; Deok Ha Woo; Seong Chan Jun; Ki-Ho Park; Seok Hwan Kim; Jae Hun Kim

doi:10.1117/12.2251585

16 May 2017 Imaging of single retinal ganglion cell with differential interference contrast microscopy (Conference Presentation)

Juyeong Oh, Yu Jeong Kim, Chul-Ki Kim, Taik Jin Lee, Mina Seo, Seok Lee, Deok Ha Woo, Seong Chan Jun, Ki-Ho Park, Seok Hwan Kim, Jae Hun Kim

Author Affiliations +

Proceedings Volume 10045, Ophthalmic Technologies XXVII; 100450N (2017) https://doi.org/10.1117/12.2251585
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Glaucoma is a progressive optic neuropathy, characterized by the selective loss of retinal ganglion cells (RGCs). Therefore, monitoring the change of number or morphology of RGC is essential for the early detection as well as investigation of pathophysiology of glaucoma. Since RGC layer is transparent and hyporeflective, the direct optical visualization of RGCs has not been successful so far. Therefore, glaucoma evaluation mostly depends on indirect diagnostic methods such as the evaluation of optic disc morphology or retinal nerve fiber layer thickness measurement by optical coherence tomography. We have previously demonstrated single photoreceptor cell imaging with differential interference contrast (DIC) microscopy. Herein, we successfully visualized single RGC using DIC microscopy. Since RGC layer is much less reflective than photoreceptor layer, various techniques including the control of light wavelength and bandwidth using a tunable band pass filter were adopted to reduce the chromatic aberration in z-axis for higher and clearer resolution. To verify that the imaged cells were the RGCs, the flat-mounted retina of Sprague-Dawley rat, in which the RGCs were retrogradely labeled with fluorescence, was observed by both fluorescence and DIC microscopies for direct comparison. We have confirmed that the cell images obtained by fluorescence microscopy were perfectly matched with cell images by DIC microscopy. As conclusion, we have visualized single RGC with DIC microscopy, and confirmed with fluorescence microscopy.

Conference Presentation

Citation Download Citation

Juyeong Oh, Yu Jeong Kim, Chul-Ki Kim, Taik Jin Lee, Mina Seo, Seok Lee, Deok Ha Woo, Seong Chan Jun, Ki-Ho Park, Seok Hwan Kim, and Jae Hun Kim "Imaging of single retinal ganglion cell with differential interference contrast microscopy (Conference Presentation)", Proc. SPIE 10045, Ophthalmic Technologies XXVII, 100450N (16 May 2017); https://doi.org/10.1117/12.2251585

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Microscopy

Digital image correlation

Luminescence

Visualization

Retinal scanning

Diagnostics

Nerve

Show All Keywords

Keywords/Phrases

Search In:

Publication Years