Multiphoton tomography of the human eye

Karsten König; Ana Batista; Tobias Hager; Berthold Seitz

doi:10.1117/12.2255151

21 February 2017 Multiphoton tomography of the human eye

Karsten König, Ana Batista, Tobias Hager, Berthold Seitz

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 1006906 (2017) https://doi.org/10.1117/12.2255151
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Multiphoton tomography (MPT) is a novel label-free clinical imaging method for non-invasive tissue imaging with high spatial (300 nm) and temporal (100 ps) resolutions. In vivo optical histology can be realized due to the nonlinear excitation of endogenous fluorophores and second-harmonic generation (SHG) of collagen. Furthermore, optical metabolic imaging (OMI) is performed by two-photon autofluorescence lifetime imaging (FLIM). So far, applications of the multiphoton tomographs DermaInspect and MPTflex were limited to dermatology. Novel applications include intraoperative brain tumor imaging as well as cornea imaging. In this work we describe two-photon imaging of ex vivo human corneas unsuitable for transplantation. Furthermore, the cross-linking (CXL) process of corneal collagen based on UVA exposure and 0.1 % riboflavin was studied. The pharmacokinetics of the photosensitizer could be detected with high spatial resolution. Interestingly, an increase in the stromal autofluorescence intensity and modifications of the autofluorescence lifetimes were observed in the human corneal samples within a few days following CXL.

Conference Presentation

Citation Download Citation

Karsten König, Ana Batista, Tobias Hager, and Berthold Seitz "Multiphoton tomography of the human eye", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006906 (21 February 2017); https://doi.org/10.1117/12.2255151

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