Development and analytical performance evaluation of FREND-SAA and FREND-Hp

Eunha Choi; Jihyun Seong; Seiyoung Lee; Changseop Lee; Sunmi Han

doi:10.1117/12.2270649

12 July 2017 Development and analytical performance evaluation of FREND-SAA and FREND-Hp

Eunha Choi, Jihyun Seong, Seiyoung Lee, Changseop Lee, Sunmi Han

Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 103240A (2017) https://doi.org/10.1117/12.2270649
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of

Abstract

The FREND System is a portable cartridge reader, quantifying analytes by measuring laser-induced fluorescence in a single-use reagent cartridge. The objective of this study was to evaluate FREND-SAA and FREND-Hp assays. The FREND-SAA and Hp assays were standardized to the WHO and IFCC reference materials. Analytical performance studies of Precision, Linearity, Limits of Detections, Interferences, and Method Comparisons for both assays were performed according to the CLSI guidelines. Both assays demonstrated acceptable imprecision of %CV in three different levels of samples. The linearity of the assays was found to be acceptable (SAA 5~150 mg/L, Hp 30~400 mg/dL). The detection limits were 3.8 mg/L (SAA) and 10.2 mg/dL (Hp). No significant interference and no significant deviation from linearity was found in the both comparison studies. In conclusion, NanoEnTek’s FREND-SAA and Hp assays represent rapid, accurate and convenient means to quantify SAA and Hp in human serum on FREND system.

Citation Download Citation

Eunha Choi, Jihyun Seong, Seiyoung Lee, Changseop Lee, and Sunmi Han "Development and analytical performance evaluation of FREND-SAA and FREND-Hp", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240A (12 July 2017); https://doi.org/10.1117/12.2270649

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KEYWORDS

Statistical analysis

Analytical research

Lung cancer

Imaging spectroscopy

Calibration

Diagnostics

Laser induced fluorescence

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