Wavefront correction for superresolution microscopy (Conference Presentation)

Peter Kner; Kayvan F. Tehrani; Benjamin Thomas; Abhijit Marar

doi:10.1117/12.2275846

29 September 2017 Wavefront correction for superresolution microscopy (Conference Presentation)

Peter Kner, Kayvan F. Tehrani, Benjamin Thomas, Abhijit Marar

Proceedings Volume 10350, Nanoimaging and Nanospectroscopy V; 1035006 (2017) https://doi.org/10.1117/12.2275846
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

Superresolution microscopy is rapidly becoming an essential tool in the biological sciences allowing imaging biological structure at length scales below 250 nm. Currently, superresolution microscopy has been applied successfully on single cells achieving resolutions of 100nm down to 20nm over a few microns of depth. When superresolution microscopy is applied in thicker samples the resolution rapidly degrades. Optical aberrations and scattering distort and reduce the point spread function causing different superresolution techniques to fail in different ways. I will discuss our work on combining structured illumination microscopy and stochastic optical reconstruction microscopy with adaptive optics to achieve sub-diffraction resolution in thick tissue.

Conference Presentation

Citation Download Citation

Peter Kner, Kayvan F. Tehrani, Benjamin Thomas, and Abhijit Marar "Wavefront correction for superresolution microscopy (Conference Presentation)", Proc. SPIE 10350, Nanoimaging and Nanospectroscopy V, 1035006 (29 September 2017); https://doi.org/10.1117/12.2275846

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available