In vivo characterizations of optical properties of human skin responses to visible light by diffuse reflectance spectroscopy (Conference Presentation)

Indermeet Kohli; Tasneem F. Mohammad M.D.; Cynthia L. Nicholson M.D.; Nikiforos Kollias; Henry W. Lim M.D.; Iltefat Hamzavi M.D.

doi:10.1117/12.2290175

14 March 2018 In vivo characterizations of optical properties of human skin responses to visible light by diffuse reflectance spectroscopy (Conference Presentation)

Indermeet Kohli, Tasneem F. Mohammad M.D., Cynthia L. Nicholson M.D., Nikiforos Kollias, Henry W. Lim M.D., Iltefat Hamzavi M.D.

Author Affiliations +

Proceedings Volume 10467, Photonics in Dermatology and Plastic Surgery 2018; 104670V (2018) https://doi.org/10.1117/12.2290175
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Solar radiation has been accepted as a major contributor to the development of skin cancer. Recent studies have shown that visible light (VL), a major portion of solar spectrum, induces biologic effects. UV filters in currently available sunscreens do not offer protection against VL. Diffuse reflectance spectroscopy (DRS) is a noninvasive objective assessment technique. Previous studies have focused on physiological parameters including melanin concentration, hemoglobin concentration, scattering and oxygen saturation. However, the differential absorbance spectra, difference between absorbance spectra of irradiated site and adjacent un-irradiated skin, can aid in the identification of various phases of skin response after VL irradiation. We irradiated the back of 62 subjects with a VL source, which consisted of a halogen lamp with filters resulting in a spectral output with greater than 98% VL. Reflectance spectra was collected for VL irradiated sites (160 J/cm2 to 480 J/cm2) immediately post-irradiation, at 24 hours, and at 7 days post-irradiation. DRS aided in classifying skin responses in terms of clinical and sub-clinical erythema accompanied by pigmentation phases based on the corresponding spectral signatures. The response in terms of skin darkness was assessed by integrating the differential absorbance spectra between 400 and 700 nm (AUC, area under the curve) since it includes contribution from both melanin and hemoglobin. The findings demonstrate the potential of DRS in assessing photoprotection offered by a product against VL induced pigmentation and erythema, VL protection factor, which can be assessed by comparing the AUC of the protected skin to that of the unprotected skin.

Conference Presentation

Citation Download Citation

Indermeet Kohli, Tasneem F. Mohammad M.D., Cynthia L. Nicholson M.D., Nikiforos Kollias, Henry W. Lim M.D., and Iltefat Hamzavi M.D. "In vivo characterizations of optical properties of human skin responses to visible light by diffuse reflectance spectroscopy (Conference Presentation)", Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670V (14 March 2018); https://doi.org/10.1117/12.2290175

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Skin

Absorbance

Visible radiation

Diffuse reflectance spectroscopy

Optical filters

In vivo imaging

Optical properties

Show All Keywords

Keywords/Phrases

Search In:

Publication Years