High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy

Jun Nie; Yusha Li; Fang Zhao; Junyu Ping; Sa Liu; Tingting Yu; Dan Zhu; Peng Fei

doi:10.1117/12.2295273

12 February 2018 High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy

Jun Nie, Yusha Li, Fang Zhao, Junyu Ping, Sa Liu, Tingting Yu, Dan Zhu, Peng Fei

Proceedings Volume 10481, Neural Imaging and Sensing 2018; 104811X (2018) https://doi.org/10.1117/12.2295273
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Light-sheet fluorescence microscopy (LSFM) uses an additional laser-sheet to illuminate selective planes of the sample, thereby enabling three-dimensional imaging at high spatial-temporal resolution. These advantages make LSFM a promising tool for high-quality brain visualization. However, even by the use of LSFM, the spatial resolution remains insufficient to resolve the neural structures across a mesoscale whole mouse brain in three dimensions. At the same time, the thick-tissue scattering prevents a clear observation from the deep of brain. Here we use multi-view LSFM strategy to solve this challenge, surpassing the resolution limit of standard light-sheet microscope under a large field-of-view (FOV). As demonstrated by the imaging of optically-cleared mouse brain labelled with thy1-GFP, we achieve a brain-wide, isotropic cellular resolution of ~3μm. Besides the resolution enhancement, multi-view braining imaging can also recover complete signals from deep tissue scattering and attenuation. The identification of long distance neural projections across encephalic regions can be identified and annotated as a result.

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Jun Nie, Yusha Li, Fang Zhao, Junyu Ping, Sa Liu, Tingting Yu, Dan Zhu, and Peng Fei "High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy", Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104811X (12 February 2018); https://doi.org/10.1117/12.2295273

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KEYWORDS

Brain

Microscopy

Neuroimaging

Brain mapping

Deconvolution

Image resolution

Microscopes

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