Macroscopic time-resolved imaging of tumor (Conference Presentation)

Vladislav I. Shcheslavskiy; Marina Shirmanova; Victoria Zherdeva; Alena Gavrina; Varvara Dudenkova; Elena Zagaynova; Alexander Savitskiy; Wolfgang Becker

doi:10.1117/12.2299317

14 March 2018 Macroscopic time-resolved imaging of tumor (Conference Presentation)

Vladislav I. Shcheslavskiy, Marina Shirmanova, Victoria Zherdeva, Alena Gavrina, Varvara Dudenkova, Elena Zagaynova, Alexander Savitskiy, Wolfgang Becker

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Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104980V (2018) https://doi.org/10.1117/12.2299317
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

This presentation describes the development of the optical macroscanner and its application for metabolic imaging of large areas of tumors in mice. The scanner allows to interrogate areas as large as 15x15mm with the lateral resolution on the order of 15 micrometers. Acquisition times range from a few seconds for low pixel numbers to several minutes for high-resolution images. We present data for NAD(P)H imaging of tumor with genetically encoded mKate2. In addition, using macroscanner we demonstrated the possibility of visualizing caspase-3 activity using the FRET-biosensor TR23K, which is based on a pair of proteins - a red fluorescent protein as a donor and a chromoprotein as an acceptor. The in vivo assay was noninvasive and could be applied in strongly and weakly fluorescent subcutaneous xenografts in mice using the FLIM-FRET method.

Conference Presentation

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Vladislav I. Shcheslavskiy, Marina Shirmanova, Victoria Zherdeva, Alena Gavrina, Varvara Dudenkova, Elena Zagaynova, Alexander Savitskiy, and Wolfgang Becker "Macroscopic time-resolved imaging of tumor (Conference Presentation)", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980V (14 March 2018); https://doi.org/10.1117/12.2299317

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KEYWORDS

Tumors

Fluorescent proteins

In vivo imaging

Proteins

Scanners

Visualization

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