Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

M. Quintavalla; P. Pozzi; Michelle Verhaegen; Hielke Bijlsma; Hans Verstraete; S. Bonora

doi:10.1117/12.2290036

23 February 2018 Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

M. Quintavalla, P. Pozzi, Michelle Verhaegen, Hielke Bijlsma, Hans Verstraete, S. Bonora

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104981X (2018) https://doi.org/10.1117/12.2290036
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.

Conference Presentation

Citation Download Citation

M. Quintavalla, P. Pozzi, Michelle Verhaegen, Hielke Bijlsma, Hans Verstraete, and S. Bonora "Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981X (23 February 2018); https://doi.org/10.1117/12.2290036

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