Practical guidelines for implementing adaptive optics in fluorescence microscopy

Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen

doi:10.1117/12.2287647

23 February 2018 Practical guidelines for implementing adaptive optics in fluorescence microscopy

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen

Proceedings Volume 10502, Adaptive Optics and Wavefront Control for Biological Systems IV; 105021F (2018) https://doi.org/10.1117/12.2287647
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.

Citation Download Citation

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, and Michel Verhaegen "Practical guidelines for implementing adaptive optics in fluorescence microscopy", Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105021F (23 February 2018); https://doi.org/10.1117/12.2287647

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