Label-free imaging of lipid droplets in cells by stimulated Raman microscopy

Maria Antonietta Ferrara; Angela Filograna; Annalisa D'Arco; Rajeev Ranjan; Michele Larobina; Carmen Valente; Luigi Sirleto

doi:10.1117/12.2317993

24 May 2018 Label-free imaging of lipid droplets in cells by stimulated Raman microscopy

Maria Antonietta Ferrara, Angela Filograna, Annalisa D'Arco, Rajeev Ranjan, Michele Larobina, Carmen Valente, Luigi Sirleto

Author Affiliations +

Proceedings Volume 10677, Unconventional Optical Imaging; 106770P (2018) https://doi.org/10.1117/12.2317993
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Recent technological developments in ultrafast laser physics have permitted to make new kind of nonlinear microscopies, as microscopy based on Stimulated Raman scattering. These techniques are based on vibrational contrast mechanism for imaging with high sensitivity, high spatial and spectral resolution and 3D sectioning capability. The interest in the study of lipids and the possibility to image lipid droplets, thanks to their isolated Raman peaks associated with vibrational C-H bond, have encouraged investigation and identification of lipid structures inside cells, taking advantage of Stimulated Raman Scattering (SRS) imaging. In this work, we report and discuss label free images on biological environmental and structural analysis, to detect lipid microstructures inside adipocyte cells.

Citation Download Citation

Maria Antonietta Ferrara, Angela Filograna, Annalisa D'Arco, Rajeev Ranjan, Michele Larobina, Carmen Valente, and Luigi Sirleto "Label-free imaging of lipid droplets in cells by stimulated Raman microscopy", Proc. SPIE 10677, Unconventional Optical Imaging, 106770P (24 May 2018); https://doi.org/10.1117/12.2317993

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