Controlled optical manipulation of particles within a buried microfluidic network  (Conference Presentation)

Lynn Paterson; Anusha Keloth; Melanie Jimenez; Ajoy K. Kar

doi:10.1117/12.2323670

17 September 2018 Controlled optical manipulation of particles within a buried microfluidic network (Conference Presentation)

Lynn Paterson, Anusha Keloth, Melanie Jimenez, Ajoy K. Kar

Author Affiliations +

Proceedings Volume 10723, Optical Trapping and Optical Micromanipulation XV; 1072316 (2018) https://doi.org/10.1117/12.2323670
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States

Abstract

A microfluidic device consisting of a 3D network of buried microchannels and integrated waveguides has been fabricated and used to controllably manipulate particles within the micro channels. The channels and waveguides were made using the direct laser writing technique of ultrafast laser inscription, followed by selective chemical etching to fabricate channels. Particles flowing through the device undergo hydrodynamic flow focusing into a narrow stream within a main channel due to the geometry of the channel network. 3D hydrodynamic focusing performance was validated using polystyrene microspheres, coloured dye and cells by visualizing the focusing within the device. A focusing width of 4 um was achieved, reducing the risk of particles sticking to walls, clogging the channel and ensuring all particles pass the beam. Particles are irradiated by 1064 nm light, in a direction perpendicular to the flow, from the embedded waveguide, causing a lateral displacement of the particle due to the optical scattering force. 5 and 10 micron beads in water were focused to a narrow stream. Lateral displacement was evaluated for 5 different laser powers for particles flowing at a constant velocity >1 mm/s. A linear increase in displacement of the particles with laser power was observed. Bacteria, yeast, microalgae and mammalian cells have been flow-focused and optically manipulated within the device. The device is capable of both passive and active separation of particle species, and the routing of particles to required outlets demonstrates potential for cell sorting.

Conference Presentation

Citation Download Citation

Lynn Paterson, Anusha Keloth, Melanie Jimenez, and Ajoy K. Kar "Controlled optical manipulation of particles within a buried microfluidic network (Conference Presentation)", Proc. SPIE 10723, Optical Trapping and Optical Micromanipulation XV, 1072316 (17 September 2018); https://doi.org/10.1117/12.2323670

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KEYWORDS

Particles

Microfluidics

Optical manipulation

Waveguides

Fabrication

Light scattering

Multiphoton lithography

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