The dynamic super-resolution phase imaging based on low-cost lensfree system

Jialin Zhang; Qian Chen; Jiaji Li; Jiasong Sun; Tianyang Ding; Chao Zuo

doi:10.1117/12.2500486

24 July 2018 The dynamic super-resolution phase imaging based on low-cost lensfree system

Jialin Zhang, Qian Chen, Jiaji Li, Jiasong Sun, Tianyang Ding, Chao Zuo

Proceedings Volume 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018); 108271F (2018) https://doi.org/10.1117/12.2500486
Event: Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 2018, Shanghai, China

Abstract

In this Letter, we present a new active micro-scanning setup and associated super-resolution (SR) phase reconstruction method in lensfree microscopy to achieve SR dynamic phase imaging. By rotating two orthogonal parallel plates to achieve controllable micro-scanning, a set of low-resolution (LR) intensity images with relative sub-pixel displacements can be acquired. These LR intensity images are then processed sequentially based on the matched imaging pixel transfer model and SR reconstruction algorithm to obtain the super-resolved quantitative phase images. The reconstructed result of Benchmark Quantitative Phase Microscopy Target (QPTTM) demonstrates the resolution enhancement quantitatively, which achieves a half-pitch lateral resolution of 775 nm over a large field-of-view (FOV) of ∼ 29.84mm² , surpassing 2.15 times of the theoretical NyquistShannon sampling resolution limit imposed by the pixel-size of the sensor (1.67 μm). Investigations of unstained Hela cells are then presented, suggesting that the method developed can provide promising application in the dynamic study as well as morphological analysis of the subcellular features for unlabelled biological samples.

Citation Download Citation

Jialin Zhang, Qian Chen, Jiaji Li, Jiasong Sun, Tianyang Ding, and Chao Zuo "The dynamic super-resolution phase imaging based on low-cost lensfree system", Proc. SPIE 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 108271F (24 July 2018); https://doi.org/10.1117/12.2500486

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