Effcient quantitative phase imaging for programmable LED light microscopy

Jiaji Li; Jialin Zhang; Qian Chen; Chao Zuo

doi:10.1117/12.2500975

24 July 2018 Effcient quantitative phase imaging for programmable LED light microscopy

Jiaji Li, Jialin Zhang, Qian Chen, Chao Zuo

Proceedings Volume 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018); 108273P (2018) https://doi.org/10.1117/12.2500975
Event: Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 2018, Shanghai, China

Abstract

We demonstrate an efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination. As a new type of coded light source, the LED array provides flexible illumination control for noninterferometric QPI based on a traditional microscopic configuration. The proposed annular LED illumination modulates the transfer function of system by changing the LED illumination pattern, which provides noiserobust response of transfer function and achieves twice resolution limit of objective NA. The quantitative phase can be recovered from slightly defocused intensity images through inversion of transfer function. Moreover, the weak object transfer function (WOTF) of axis-symmetric oblique source is derived, and the noise-free and noisy simulation results validate the predicted theory. Finally, we experimentally confirm accurate and repeatable performance of our method by imaging cellular specimens with different NA objectives.

Citation Download Citation

Jiaji Li, Jialin Zhang, Qian Chen, and Chao Zuo "Effcient quantitative phase imaging for programmable LED light microscopy", Proc. SPIE 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 108273P (24 July 2018); https://doi.org/10.1117/12.2500975

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KEYWORDS

Objectives

Light emitting diodes

Image resolution

LED lighting

Phase imaging

Phase transfer function

Microscopy

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