Hyperspectral digital holographic microscopy approach for reduction of coherence induced disturbances in quantitative phase imaging of biological specimens

Álvaro Barroso; Steffi Ketelhut; Peter Heiduschka; Lena Kastl; Jürgen Schnekenburger; Björn Kemper

doi:10.1117/12.2319179

7 September 2018 Hyperspectral digital holographic microscopy approach for reduction of coherence induced disturbances in quantitative phase imaging of biological specimens

Álvaro Barroso, Steffi Ketelhut, Peter Heiduschka, Lena Kastl, Jürgen Schnekenburger, Björn Kemper

Author Affiliations +

Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 108340I (2018) https://doi.org/10.1117/12.2319179
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland

Abstract

Coherence induced noise and parasitic reflections in the experimental setup are main restrictions that limit the resolution and measurement accuracy in laser light-based digital holographic microscopy (DHM). We explored, if coherence properties of partial coherent light sources can be mimicked by utilizing spectrally tunable lasers. Moreover, the performance for label-free quantitative phase imaging of biological specimens is illustrated utilizing an experimental configuration including a commercial microscope and tunable super continuum laser sources with a wavelength range of up to 230 nm.

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Álvaro Barroso, Steffi Ketelhut, Peter Heiduschka, Lena Kastl, Jürgen Schnekenburger, and Björn Kemper "Hyperspectral digital holographic microscopy approach for reduction of coherence induced disturbances in quantitative phase imaging of biological specimens", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108340I (7 September 2018); https://doi.org/10.1117/12.2319179

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