Raman hyperspectral imaging of different salivary gland cell types for tissue engineering

Ting Chean Khoo; Nicholas Moskwa; Anna Sharikova; Melinda Larsen; Alexander Khmaladze

doi:10.1117/12.2511396

4 March 2019 Raman hyperspectral imaging of different salivary gland cell types for tissue engineering

Ting Chean Khoo, Nicholas Moskwa, Anna Sharikova, Melinda Larsen, Alexander Khmaladze

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108901S (2019) https://doi.org/10.1117/12.2511396
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Regenerative medicine encompasses the rebuilding or repairing of organs. We are developing bioengineered organoids that will differentiate when implanted in vivo to partially restore organ function. These complex organoids, derived from embryonic salivary gland cells, include both primary mesenchyme and epithelial progenitor cells. Noninvasive quality monitoring of tissue-engineered constructs is required before implantation of bioengineered constructs in vivo. Raman spectroscopy offers fast, simple, and, most importantly, non-invasive quantitative cell and tissue analysis that does not require elaborate sample preparation. We demonstrate the application of Raman micro-spectroscopy technique to in vitro monitoring of cell types within 3D cell clusters, with the ultimate goal of applying this technology in situ to monitor adult cell-derived organoids that are implanted in vivo. We have collected Raman spectra of epithelial and mesenchymal progenitor cells in vitro, and have shown that we are able to identify different Raman signatures corresponding to each cell type. In particular, we have observed Raman spectral differences which correspond to the C-C and C-N stretch in proteins, as well as in the Amide I and III envelopes. The embryonic mesenchyme cells are similar to mesenchymal stem cells, MSCs, which can differentiate into bone, cartilage, and other cell types. In addition to salivary gland tissue engineering applications, mesenchymal cells offer a great potential in repairing bone, cartilage, and damaged heart cells, and to treat inflammation and immune system diseases. In future studies, our Raman spectroscopy methods can be broadly applied to monitoring of organoids for application in many diseases.

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Ting Chean Khoo, Nicholas Moskwa, Anna Sharikova, Melinda Larsen, and Alexander Khmaladze "Raman hyperspectral imaging of different salivary gland cell types for tissue engineering ", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108901S (4 March 2019); https://doi.org/10.1117/12.2511396

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KEYWORDS

Raman spectroscopy

Tissues

In vivo imaging

Proteins

Tissue engineering

Regenerative medicine

Hyperspectral imaging

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