Progress in OCT based functional cellular resolution retinal imaging in mice: application of temporal super averaging

Robert J. Zawadzki; Pengfei Zhang; Eric B. Miller; Suman K. Manna; Ratheesh K. Meleppat; Marie E. Burns; Edward N. Pugh Jr.

doi:10.1117/12.2530955

19 July 2019 Progress in OCT based functional cellular resolution retinal imaging in mice: application of temporal super averaging

Robert J. Zawadzki, Pengfei Zhang, Eric B. Miller, Suman K. Manna, Ratheesh K. Meleppat, Marie E. Burns, Edward N. Pugh Jr.

Author Affiliations +

Proceedings Volume 11078, Optical Coherence Imaging Techniques and Imaging in Scattering Media III; 110780J (2019) https://doi.org/10.1117/12.2530955
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Over the last two decades optical coherence tomography (OCT) has become a clinical gold standard in diagnostic and management of many retinal diseases. Following that success, OCT is now becoming a standard tool in studies of animal models of human retinal diseases allowing longitudinal evaluation of disease progression and responses to therapeutic intervention in the single animal. Recent progress in OCT data acquisition and processing allowed successful cellular resolution detection of outer and inner retina functional response to light stimulation both in humans and experimental animals. In this manuscript, we will describe our latest effort of improving cellular contrast in mouse functional retinal imaging by application of volumetric registration and speckle contrast reduction by temporal averaging of volumetric data sets: i.e., temporal Super Averaging. As an example, the current configuration of our multimodal Scanning Light Ophthalmoscopy / Optical Coherence Tomography (SLO/OCT) mice retinal imaging system, as well as data acquisition and processing schemes, will be presented. Experimental results will be discussed in the context of improved morphological and functional probing of mice retinas at cellular resolution. Finally, current limitations and future directions of instrument development with a focus on improved probing of the retinal function will be discussed as well.

Citation Download Citation

Robert J. Zawadzki, Pengfei Zhang, Eric B. Miller, Suman K. Manna, Ratheesh K. Meleppat, Marie E. Burns, and Edward N. Pugh Jr. "Progress in OCT based functional cellular resolution retinal imaging in mice: application of temporal super averaging ", Proc. SPIE 11078, Optical Coherence Imaging Techniques and Imaging in Scattering Media III, 110780J (19 July 2019); https://doi.org/10.1117/12.2530955

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical coherence tomography

Retinal scanning

Image resolution

Speckle

Image registration

Eye

Adaptive optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years