Imaging depth extension of optical coherence tomography by optical clearing method in vitro rabbit eye

Ruiming Kong; Wenjuan Wu; Hongwei Zhao; Rui Qiu; Guoqiang Li; Ailin Liu; Xinmin Lu; Cuixia Dai

doi:10.1117/12.2537551

20 November 2019 Imaging depth extension of optical coherence tomography by optical clearing method in vitro rabbit eye

Ruiming Kong, Wenjuan Wu, Hongwei Zhao, Rui Qiu, Guoqiang Li, Ailin Liu, Xinmin Lu, Cuixia Dai

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111900R (2019) https://doi.org/10.1117/12.2537551
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Optical coherence tomography (OCT) is a noninvasive, non-contact imaging technique, which is an indispensable diagnostic tool in ophthalmology. However, light attenuation in opaque tissues of the eye limits the imaging depth of OCT. In this study, the imaging depth of rabbit eye in vitro was extended by reduction of multiple scattering by using tissue optical clearing (TOC) agents. Results showed that with the application of glycerol in anterior segment, boundaries of ciliary muscle could be obviously differentiated. For posterior segment, the retina, the choroid, the sclera and even the eye container were also clearly imaged. All the physiology change by glycerol was recovered by a direct wash of saline in a short time.

Citation Download Citation

Ruiming Kong, Wenjuan Wu, Hongwei Zhao, Rui Qiu, Guoqiang Li, Ailin Liu, Xinmin Lu, and Cuixia Dai "Imaging depth extension of optical coherence tomography by optical clearing method in vitro rabbit eye", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111900R (20 November 2019); https://doi.org/10.1117/12.2537551

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available