Imaging cancer-associated fibroblasts in human breast tumor tissue using multiphoton microscopy

Liqin Zheng; Wenhui Guo; Gangqin Xi; Jiajia He; Wenjiao Ren; Deyong Kang; Biying Yu; Chuan Wang; Jianxin Chen

doi:10.1117/12.2546962

20 December 2019 Imaging cancer-associated fibroblasts in human breast tumor tissue using multiphoton microscopy

Liqin Zheng, Wenhui Guo, Gangqin Xi, Jiajia He, Wenjiao Ren, Deyong Kang, Biying Yu, Chuan Wang, Jianxin Chen

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112091G (2019) https://doi.org/10.1117/12.2546962
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

Fibroblasts are important players in proliferation, invasion, migration and metastasis of cancer cells. Until now, histological methods such as hematoxylin and eosin (H and E) staining are still a gold standard to assess fibroblasts in traditional histopathology. Multiphoton microscopy (MPM) has become a key technology for biological tissues imaging without staining at the cellular level. In this study, MPM based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was used to identify the fibroblasts in breast cancer tissue. Normal fibroblasts with spindle shape and abnormal fibroblasts with stellate shape could be clearly obtained by MPM.

Citation Download Citation

Liqin Zheng, Wenhui Guo, Gangqin Xi, Jiajia He, Wenjiao Ren, Deyong Kang, Biying Yu, Chuan Wang, and Jianxin Chen "Imaging cancer-associated fibroblasts in human breast tumor tissue using multiphoton microscopy", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112091G (20 December 2019); https://doi.org/10.1117/12.2546962

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