A facile way to synthesize dual-drug delivery nanocarrier for highly efficient therapy and tracing of intracellular drug release

Peng Chen; Wenzhe Li; Zhuyuan Wang; Yiping Cui

doi:10.1117/12.2550612

20 December 2019 A facile way to synthesize dual-drug delivery nanocarrier for highly efficient therapy and tracing of intracellular drug release

Peng Chen, Wenzhe Li, Zhuyuan Wang, Yiping Cui

Author Affiliations +

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112095J (2019) https://doi.org/10.1117/12.2550612
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

Dual-drug delivery systems designed for effective therapy is receiving much attention in cancer treatment, however, monitoring of the stimuli-responsive multi-drug release dynamics in living cells is still challenging. Here, a dual drug nanocarrier has been successfully synthesized using functionalized carbon nanotubes (CNTs). To obtain effective combination therapy, CNTs are used for loading dual drugs, that is, 6-thioguanine (6TG) and doxorubicin (DOX).By using label-free surface enhanced Raman scattering (SERS) and fluorescence techniques, the release dynamics of the loaded two kinds of drugs can be traced in living cells. In this nanocarrier, 6TG was conjugated to the surfaces of gold-silver core-shell nanoparticles (Au@Ag NPs). Release of 6TG can be triggered by glutathione (GSH). DOX was loaded onto CNTs surfaces via π–π stacking, which exhibits a pH-responsive release behavior. To investigate the intracellular traceable delivery performance of this nanocarrier, the dual drug loaded nanocarrier was incubated with living HeLa cells. Experimental results indicated that this dual drug nanocarrier can effectively enter into HeLa cells. And the release of 6TG and DOX were triggered by the substation of GSH and the acidic environment of lysosomes, respectively. By recording the real-time SERS and fluorescence signals of dual-drug inside HeLa cells, we can successfully monitor the dynamic process of intracellular drug release. Moreover, the combination of 6TG and DOX exhibited a synergistic effect to enhance the efficacy of cancer therapy. This kind of dual drug nanocarrier presents a new perspective for the design of smart multi-drug delivery systems for cancer therapy.

Citation Download Citation

Peng Chen, Wenzhe Li, Zhuyuan Wang, and Yiping Cui "A facile way to synthesize dual-drug delivery nanocarrier for highly efficient therapy and tracing of intracellular drug release", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112095J (20 December 2019); https://doi.org/10.1117/12.2550612

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Nanoparticles

Cancer

Absorption

Gold

Carbon nanotubes

Fluorescence spectroscopy

Surface enhanced Raman spectroscopy

Keywords/Phrases

Search In:

Publication Years