Evaluation of low level laser therapy, platelet rich plasma, and their combination on the proliferation rate of human periodontal ligament fibroblast: an in vitro study

Shelly Ahuja; Pankaj Madhukar; Kishore Bhatt

doi:10.1117/12.2543331

19 February 2020 Evaluation of low level laser therapy, platelet rich plasma, and their combination on the proliferation rate of human periodontal ligament fibroblast: an in vitro study

Shelly Ahuja, Pankaj Madhukar, Kishore Bhatt

Author Affiliations +

Proceedings Volume 11217, Lasers in Dentistry XXVI; 1121709 (2020) https://doi.org/10.1117/12.2543331
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The major cellular events in the tissue repair are mitogenesis, migration and metabolism. The proteins responsible for coordination of these events are called “growth factors". Activated platelets at the wound margins release several growth factors, such as PDGF, TFG-β and EGF, etc. The current study was conducted to determine whether there are additional effects of PRP combined Laser therapy. In this study PDL cells were obtained during 3rd molar impaction surgery, these cells were cultured under standard conditions and PDLF were spread on tissue culture plates. Platelet concentrate was obtained after centrifugation and 15ml of platelet concentrate was added to each well. Subconfluent monolayer’s were irradiated with an 904nm GaAs laser operated at a power output of 14mv in a continuous wave mode at energy fluences of 4.02J/cm², frequency 10,000 Hz. Time of exposure was 300 sec per well and number of irradiation were 3. After every laser treatment, the culture was incubated for 24hrs. The proliferation rate was determined by nonradioactive assay containing redox indicator with Alamar Blue Dye after 72 hrs. The numbers of cells were counted under neubar counting chamber after 24, 48 and 72 hrs. The results showed that the treatment of fibroblast with LLLT + PRP revealed a considerably higher proliferation activity than control group. The differences were significantly upto 72 hrs (Mann Whitney U test,p<0.001) A cellular effect of the laser and platelet concentrate is clearly discernible. It was concluded that the use of platelet conc.and laser is an effective modality of regeneration.

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Citation Download Citation

Shelly Ahuja, Pankaj Madhukar, and Kishore Bhatt "Evaluation of low level laser therapy, platelet rich plasma, and their combination on the proliferation rate of human periodontal ligament fibroblast: an in vitro study", Proc. SPIE 11217, Lasers in Dentistry XXVI, 1121709 (19 February 2020); https://doi.org/10.1117/12.2543331

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