Quantification of infrared spectral markers for ulcerative colitis using sera

A. G. Unil Perera; Hemendra Ghimire

doi:10.1117/12.2545096

21 February 2020 Quantification of infrared spectral markers for ulcerative colitis using sera

A. G. Unil Perera, Hemendra Ghimire

Proceedings Volume 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry; 112360T (2020) https://doi.org/10.1117/12.2545096
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

This study investigates an application of Fourier transform infrared (FTIR) spectroscopy of blood components in attenuated total reflection (ATR) sampling mode to quantify ulcerative colitis (UC) induced molecular alterations. Using infrared absorbance data of serum samples extracted from Interleukin 10 knockout (IL10-/-) and Dextran Sodium Sulfate (DSS)-induced experimental models of colitis, we have quantified associated markers with the aid of accompanying data analysis techniques. Identified spectral markers are absorbance at wavenumber 1033 cm^-1 , which is primarily due to the glucose; 1076 cm^-1 , representing mannose as well as phosphate presence; and the ratio of absorbance at 1121 cm^-1 RNA presence to its value at 1020 cm^-1 , associated with DNA; and at 1629 cm^-1 belonging to the protein to its value at 1737 cm^-1 belonging to lipids’ presence. Protein secondary structures as observed by spectral deconvolution in the Amide-I band was also identified. The quantified discriminatory values show significant fluctuations in colitis samples compared to their control types. The differentiating signatures between spectra are obtained by observing p-values comparisons, the ratio analysis and the use of statistical measures such as sensitivity and specificity. High diagnostic accuracy is seen with 80-100% sensitivity and specificity values. Thus, quantitative analysis of infrared (IR) spectral data may be useful for disease diagnostics and therapeutic analysis.

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A. G. Unil Perera and Hemendra Ghimire "Quantification of infrared spectral markers for ulcerative colitis using sera", Proc. SPIE 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry, 112360T (21 February 2020); https://doi.org/10.1117/12.2545096

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KEYWORDS

Decision support systems

Statistical analysis

Absorbance

Infrared radiation

Proteins

Diagnostics

Spectroscopy

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