Evaluation of newly synthesized potential NLO-phores for 2-photon and SHG imaging

A. Marcek Chorvatova; M. Uherek; I. Sigmundova; P. Magdolen; D. Chorvat

doi:10.1117/12.2543350

14 February 2020 Evaluation of newly synthesized potential NLO-phores for 2-photon and SHG imaging

A. Marcek Chorvatova, M. Uherek, I. Sigmundova, P. Magdolen, D. Chorvat

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124410 (2020) https://doi.org/10.1117/12.2543350
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Our contribution is focused on broadening of the spectrum of available non-linear optical (NLO)-phores (contrast agents for nonlinear optical microscopy) by design and synthesis of new organic dyes with appropriate optical properties. One of the main pre-requisites of microscopy utilizing non-linear excitation is the existence of molecules that are able to provide NLO response for the second-harmonic generation (SHG) or for the two-photon excited fluorescence (TPEF). Many molecules naturally occurring in living tissue such as collagens or NAD(P)H were successfully used in this regard, but there is a natural interest in broadening of the spectrum of available NLO-phores. Gathered results confirm applicability of the newly synthesized dyes as new potential NLO-phores for confocal laser scanning microscopy with nonlinear excitation in rat aorta.

Conference Presentation

Citation Download Citation

A. Marcek Chorvatova, M. Uherek, I. Sigmundova, P. Magdolen, and D. Chorvat "Evaluation of newly synthesized potential NLO-phores for 2-photon and SHG imaging", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124410 (14 February 2020); https://doi.org/10.1117/12.2543350

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